Luotonen J
Acta Otolaryngol. 1982;93(1-6):295-9. doi: 10.3109/00016488209130886.
Middle ear fluid (MEF) aspirates and nasal swabs of children with acute otitis media (N = 255, age 2-6 yrs) were cultured by standard bacteriological methods. In addition, counterimmunoelectrophoresis and latex agglutination were applied to detect pneumococcal polysaccharide antigens (Pn-ag) in MEF. When the nasal culture grew Streptococcus pneumoniae (Pn), Pn was also present in 49% (73/148) and Pn-ag in 64% of the MEF cultures. If Haemophilus influenzae (Hi) appeared in the nasal culture. Hi was present in 41% (25/61) of the MEFs. There was a negative correlation between the growth of Pn or Hi in the nasal culture and the presence of other bacteria in the MEF. However, in spite of these correlations the nasal culture was concluded not to be useful in predicting the MEF bacteria.
采用标准细菌学方法对255名（年龄在2至6岁之间）患有急性中耳炎儿童的中耳积液（MEF）抽吸物和鼻拭子进行培养。此外，应用对流免疫电泳和乳胶凝集试验检测MEF中的肺炎球菌多糖抗原（Pn-ag）。当鼻拭子培养出肺炎链球菌（Pn）时，49%（73/148）的MEF培养物中也存在Pn，64%的MEF培养物中存在Pn-ag。如果鼻拭子培养出流感嗜血杆菌（Hi），41%（25/61）的MEF中存在Hi。鼻拭子培养中Pn或Hi的生长与MEF中其他细菌的存在呈负相关。然而，尽管存在这些相关性，但得出的结论是鼻拭子培养对于预测MEF中的细菌并无用处。
