Milković K, Peruzović M, Paunović J
Biol Neonate. 1982;41(1-2):32-7. doi: 10.1159/000241513.
One half of each litter of the 2- and 7-day-old rats was injected twice daily for 3 consecutive days with 15 mg/100 g body weight of metopirone (MET) in 0.05 ml of saline, and the remaining littermates with saline only. At the ages of 5 and 10 days, one half of the pups was sacrificed immediately, and the second half 20 min after s.c. injection of histamine as a stressor. MET depressed body weight and induced adrenal hypertrophy in the 5- and 10- day-old rats, and pituitary hypertrophy in the 5-day-old rats only. In resting condition the adrenal glands of the 5-day-old rats treated with MET contained less corticosterone (determined fluorometrically) than the 5-day-old control and the 10-day-old pups treated also with MET. Furthermore, neonatal MET treatment induced (histamine) stress response by the increase in adrenal corticosterone content in the 10-day-old rats, and by the increase in corticosterone content in plasma in both ages. The results indicate high sensitivity of the young neonatal rats to hormonal manipulations. They also yield additional support to the concept of functional integrity of the hypothalamo-pituitary-adrenocortical system in neonatal rats, and to the flexibility of the postnatal developmental pattern of stress response.
将2日龄和7日龄大鼠每窝中的一半，连续3天每天注射2次，每次注射15 mg/100 g体重的美替拉酮（MET），溶剂为0.05 ml生理盐水，其余同窝大鼠仅注射生理盐水。在5日龄和10日龄时，将一半幼崽立即处死，另一半在皮下注射组胺作为应激源20分钟后处死。美替拉酮使5日龄和10日龄大鼠体重减轻并导致肾上腺肥大，仅使5日龄大鼠垂体肥大。在静息状态下，用美替拉酮处理的5日龄大鼠肾上腺中所含的皮质酮（荧光法测定）少于5日龄对照大鼠以及同样用美替拉酮处理的10日龄幼崽。此外，新生期美替拉酮处理通过使10日龄大鼠肾上腺皮质酮含量增加以及使两个年龄段大鼠血浆中皮质酮含量增加，诱导（组胺）应激反应。结果表明新生幼鼠对激素操作高度敏感。它们还进一步支持了新生大鼠下丘脑 - 垂体 - 肾上腺皮质系统功能完整性的概念，以及出生后应激反应发育模式的灵活性。
