Wolf P L, Ferrone S
Clin Biochem. 1982 Feb;15(1):24-7. doi: 10.1016/s0009-9120(82)90367-8.
Human melanoma cells have unexpectedly been found to express Ia-like antigens, histocompatibility type antigens which are believed to be the gene products of the immune response region. Melanoma derived Ia-like antigens are immunologically functional. Monoclonal antibodies have been produced to these antigens. The level of Ia-like antigens on melanoma cells is significantly lower than on B lymphoid cells, and this is more likely to reflect a reduced synthesis than an increased shedding. We have employed radioimmunometric and immunofluorescent procedures in this investigation, Melanoma cell-derived Ia-like antigens have a structure similar to that of B lymphoid cell-derived Ia-like antigens. The antigens are composed of two non covalently associated glycoproteins. One chain, referred to as the alpha chain, has an approximate molecular weight of 34,000 and the other one, referred to as the beta chain, has an approximate molecular weight of 29,000. The expression of Ia-like antigens is restricted to malignant melanoma and not on benign nevi.
人们意外地发现，人类黑色素瘤细胞表达Ia样抗原，即组织相容性Ⅱ类抗原，据信这是免疫反应区的基因产物。黑色素瘤衍生的Ia样抗原具有免疫功能。针对这些抗原已制备出单克隆抗体。黑色素瘤细胞上Ia样抗原的水平明显低于B淋巴细胞，这更可能反映的是合成减少而非脱落增加。在本研究中，我们采用了放射免疫测定和免疫荧光方法。黑色素瘤细胞衍生的Ia样抗原与B淋巴细胞衍生的Ia样抗原结构相似。这些抗原由两种非共价结合的糖蛋白组成。一条链称为α链，分子量约为34000，另一条链称为β链，分子量约为29000。Ia样抗原的表达仅限于恶性黑色素瘤，而在良性痣中不表达。
