Schuetz A W, Rock J
Differentiation. 1982;21(1):41-4. doi: 10.1111/j.1432-0436.1982.tb01193.x.
Follicular fluid was collected from individual human ovarian follicles and its effects, alone or in combination with frog pituitary homogenate (FPH), on oocyte maturation and ovulation were assessed following incubation with amphibian ovarian follicles in vitro. Oocyte maturation, with little or no concomitant ovulation, was induced by variable amounts of follicular fluid. Some of the individual follicular fluid samples were very active in inducing oocyte maturation, whereas others were inactive. Frog pituitary homogenates exhibited biologic activity (induced oocyte maturation and ovulation) when incubated in the presence of most follicular fluid samples. However, follicular fluid samples from two individuals inhibited ovulation but not maturation in FPH-treated follicles. These results demonstrate that amphibian follicles remain viable and undergo a number of physiologic changes in the presence of unfractionated human follicular fluid. Under appropriate conditions both stimulatory and inhibitory effects of follicular fluid were observed. These data suggest that amphibian ovarian follicles may provide a simple and independent means for detecting and assaying a number of biologic activities present in follicular fluid obtained from single human and other mammalian ovarian follicles. Such results may provide the basis for dissociating endocrine and cellular interactions which occur during normal and abnormal follicular differentiation.
从人类个体卵巢卵泡中收集卵泡液，并在体外与两栖类卵巢卵泡共同孵育后，评估其单独或与蛙垂体匀浆（FPH）联合对卵母细胞成熟和排卵的影响。不同量的卵泡液可诱导卵母细胞成熟，同时伴有极少或无排卵现象。部分个体卵泡液样本在诱导卵母细胞成熟方面非常活跃，而其他样本则无活性。当与大多数卵泡液样本共同孵育时，蛙垂体匀浆表现出生物活性（诱导卵母细胞成熟和排卵）。然而，来自两名个体的卵泡液样本在FPH处理的卵泡中抑制排卵，但不抑制成熟。这些结果表明，在未分级的人类卵泡液存在的情况下，两栖类卵泡仍保持活力并经历一系列生理变化。在适当条件下，观察到了卵泡液的刺激和抑制作用。这些数据表明，两栖类卵巢卵泡可能为检测和分析从单个人类及其他哺乳动物卵巢卵泡获得的卵泡液中存在的多种生物活性提供一种简单且独立的方法。这些结果可能为分离正常和异常卵泡分化过程中发生的内分泌和细胞相互作用提供基础。
