Onoda T, Morikawa S, Harada T, Suzuki Y, Inoue K, Nishigami K
Cancer Res. 1982 Jul;42(7):2867-71.
D-Mannosamine is toxic to human malignant T-lymphoid cell lines derived from patients with T-cell leukemia. We observed heterogeneity of mannosamine susceptibility among those cell lines. The leukemic T-cell lines, subgrouped according to the degree of mannosamine inhibition on nucleic acid biosyntheses, were: Subgroup 1, HPB-MLT cells; Subgroup 2, CCRF-HSB-2 and HPB-ALL cells; and Subgroup 3, MOLT-4 cells. The most sensitive line, HPB-MLT, originated from the patient with adult T-cell leukemia. The cytotoxicity of mannosamine was potentiated by a fatty acid, sodium oleate, at concentrations that were noncytolytic, and the interaction between the two drugs was synergistic. These results would suggest that mannosamine induces changes in the membrane structure of the leukemia cells. Thus, the primary target of the tumoricidal activity of mannosamine may also be the cellular membranes.
D-甘露糖胺对源自T细胞白血病患者的人恶性T淋巴细胞系有毒性。我们观察到这些细胞系对甘露糖胺的敏感性存在异质性。根据甘露糖胺对核酸生物合成的抑制程度分组的白血病T细胞系如下：第1组，HPB-MLT细胞；第2组，CCRF-HSB-2和HPB-ALL细胞；第3组，MOLT-4细胞。最敏感的细胞系HPB-MLT源自成年T细胞白血病患者。在非细胞溶解浓度下，脂肪酸油酸钠可增强甘露糖胺的细胞毒性，且两种药物之间的相互作用是协同的。这些结果表明，甘露糖胺可诱导白血病细胞膜结构的变化。因此，甘露糖胺杀肿瘤活性的主要靶点也可能是细胞膜。
