一种用于HLA - DR抗原α链和β链的高产率纯化方法。
A high yield purification procedure for alpha and beta chains of HLA-DR antigens.
作者信息
Walker L E, Reisfeld R A
出版信息
J Biol Chem. 1982 Jul 25;257(14):7940-3.
PMID:6979540
Abstract
A procedure is described for purifying to homogeneity the alpha and beta chains of HLA-DR antigen from the homozygous human lymphoid cell line LG-2. The procedure involves a combination of lectin affinity as well as classical and high pressure liquid chromatography. From 100 g of packed cells, 8 mg (235 nmol) of alpha chain and 7 mg (205 nmol) of beta chain were isolated.
摘要
本文描述了一种从纯合子人淋巴细胞系LG-2中纯化HLA-DR抗原α链和β链至均一性的方法。该方法结合了凝集素亲和色谱以及经典和高压液相色谱。从100克 packed cells中分离出了8毫克（235纳摩尔）的α链和7毫克（205纳摩尔）的β链。 （这里“packed cells”不太明确准确中文，可根据实际情况进一步确定准确表述，比如“压实细胞”等 ）
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