猪体内B淋巴细胞及其亚群的评估。使用全血的方法与使用Ficoll-Paque纯化单核细胞的方法之间的比较。

The estimation of B lymphocytes and their subpopulations in pigs. A comparison between a method using whole blood and one using Ficoll-Paque purified mononuclear cells.

作者信息

McCauley I, Hartmann P E

出版信息

J Immunol Methods. 1982;50(1):33-8. doi: 10.1016/0022-1759(82)90301-5.

Abstract

This paper describes a simple method for determining the proportion of B lymphocytes and their subtypes in pigs, using washed whole blood. The method was compared with one using Ficoll-Paque purified mononuclear cells and was found to give significantly lower results (13.0 +/- 1.3% and 17.0 +/- 1.8%, mean +/- S.E., respectively). For 30 normal pigs, 13.9 +/- 6% (mean +/- S.D.) of lymphocytes in whole blood bore light chains and 12.1 +/- 5% bore IgM, while of lymphocytes in purified mononuclear cells, 17.0 +/- 6% bore light chains, 15.1 +/- 6% bore IgM, 1.4 +/- 1.4% bore IgG and 0.5 +/- 0.5% bore IgA.

摘要

本文描述了一种使用洗涤全血来测定猪体内B淋巴细胞及其亚型比例的简单方法。该方法与使用Ficoll-Paque纯化单核细胞的方法进行了比较，结果发现其得出的结果显著更低（分别为13.0±1.3%和17.0±1.8%，均值±标准误）。对于30头正常猪，全血中13.9±6%（均值±标准差）的淋巴细胞带有轻链，12.1±5%带有IgM，而在纯化单核细胞中的淋巴细胞，17.0±6%带有轻链，15.1±6%带有IgM，1.4±1.4%带有IgG，0.5±0.5%带有IgA。