Shibuya E K, Masui Y
J Exp Zool. 1982 May 1;220(3):381-5. doi: 10.1002/jez.1402200314.
Suspensions of lysolecithin-treated sperm were injected into blastomeres of two-cell embryos which had been arrested with the extract of unfertilized eggs containing cytostatic factor (CSF). Whereas the CSF-arrested blastomeres remained unchanged if no sperm were injected, those injected with sperm formed abortive cleavage furrows. The injected sperm exhibited characteristics similar to those associated with sperm injected into activated eggs. These were nuclear decondensation to the pronucleus, DNA synthesis, and late chromosome condensation to the metaphase stage. The activity of CSF-arrested blastomere cytoplasm to promote meiotic maturation when injected into ovarian oocytes rapidly decreased following sperm injection, but later fluctuated at low levels. It was suggested that the CSF-arrested blastomeres retained the potential to reinitiate cell cycle activities which could be evoked by sperm injection.
将经溶血卵磷脂处理的精子悬浮液注射到用含有细胞静止因子（CSF）的未受精卵提取物阻滞的二细胞胚胎的卵裂球中。如果不注射精子，CSF阻滞的卵裂球保持不变，而注射了精子的卵裂球则形成了异常的分裂沟。注射的精子表现出与注入活化卵中的精子相似的特征。这些特征包括细胞核解聚形成原核、DNA合成以及后期染色体凝聚到中期阶段。当注射到卵巢卵母细胞中时，CSF阻滞的卵裂球细胞质促进减数分裂成熟的活性在精子注射后迅速下降，但随后在低水平波动。有人提出，CSF阻滞的卵裂球保留了重新启动细胞周期活动的潜力，这种潜力可由精子注射诱发。
