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牛胸腺淋巴细胞增强的E花环形成与胸苷的自发掺入之间的相关性。

Correlation between enhanced E-rosette formation and spontaneous incorporation of thymidine by bovine thymus lymphocytes.

作者信息

Higgins D A, Stack M J

出版信息

Vet Immunol Immunopathol. 1981 Jun;2(3):265-71. doi: 10.1016/0165-2427(81)90027-1.

DOI:10.1016/0165-2427(81)90027-1
PMID:6979826
Abstract

Bovine thymus lymphocytes were centrifuged at low speed through 27% BSA and separated into five equal fractions. Enhanced formation of E rosettes and 10-20 fold increases in spontaneous uptake of tritiated (3H) thymidine occurred among the buoyant cell populations with less or no increases among cells from lower fractions. It is suggested that BSA stimulates blastogenesis and that the increases capacity for E-rosette formation is due to cell surface alterations among the activated cells or cells affected by their products.

摘要

将牛胸腺淋巴细胞通过27%的牛血清白蛋白(BSA)进行低速离心,分离成五个相等的部分。在浮力较大的细胞群体中,E花环的形成增强,氚标记(3H)胸腺嘧啶核苷的自发摄取增加了10 - 20倍,而较低部分的细胞中增加较少或没有增加。这表明BSA刺激细胞分裂,并且E花环形成能力的增加是由于活化细胞或受其产物影响的细胞的细胞表面改变。

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