表皮生长因子可诱导HeLa细胞和3T3细胞中的中心体快速分离。
Epidermal growth factor induces rapid centrosomal separation in HeLa and 3T3 cells.
作者信息
Sherline P, Mascardo R N
出版信息
J Cell Biol. 1982 May;93(2):507-12. doi: 10.1083/jcb.93.2.507.
Abstract
Using indirect immunofluorescence, we have found that epidermal growth factor (EGF), at 100 ng/ml, induces centrosomal separation within 20 min in HeLa and 3T3 cells. The effect was evident both in unsynchronized cultures and in HeLa cells blocked in early S phase by hydroxyurea. EGF also induced centrosomal separation in quiescent 3T3 cells blocked in G0/G1 by serum deprivation, indicating that DNA replication is not necessary for this effect. The mechanism of this rapid centrosomal separation and its role in the mitogenic effects of EGF remains to be determined.
摘要
利用间接免疫荧光法，我们发现，在HeLa细胞和3T3细胞中，100 ng/ml的表皮生长因子（EGF）在20分钟内即可诱导中心体分离。这种效应在未同步化的培养物以及被羟基脲阻滞于早S期的HeLa细胞中均很明显。EGF还可诱导因血清剥夺而阻滞于G0/G1期的静止3T3细胞发生中心体分离，这表明DNA复制对于该效应并非必需。这种快速的中心体分离机制及其在EGF促有丝分裂效应中的作用仍有待确定。
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