采用高效液相色谱荧光检测法测定服用复方磺胺多辛或复方磺胺多辛-甲氟喹后人体血浆中的乙胺嘧啶。

Determination of pyrimethamine in human plasma after administration of fansidar of fansidar-mefloquine by means of high-performance liquid chromatography with fluorescence detection.

作者信息

Timm U, Weidekamm E

出版信息

J Chromatogr. 1982 Jun 11;230(1):107-14. doi: 10.1016/s0378-4347(00)81435-8.

Abstract

A sensitive, rapid and selective high-performance liquid chromatographic (HPLC) method has been developed to measure plasma levels of pyrimethamine in human subjects dosed with the antimalarials Fansidar or Fansidar and mefloquine. The drug was extracted from plasma at basic pH with n-butyl chloride-dichloromethane (96:4, v/v) and quantified on a normal-phase HPLC column with fluorescence detection (excitation 290 nm, emission 345 nm). Pyrimethamine was almost quantitatively extracted from plasma in the concentration range 20-200 ng/ml. The sensitivity limit was about 10 ng/ml of plasma, using a 0.5-ml specimen. The method was shown to be specific with respect to the other two components in the antimalarial combinations, namely sulfadoxine and mefloquine, and their metabolites. The assay was applied to pharmacokinetic studies of pyrimethamine in man following the oral administration of Fansidar of Fansidar and mefloquine.

摘要

已开发出一种灵敏、快速且选择性高的高效液相色谱（HPLC）方法，用于测定服用抗疟药 Fansidar 或 Fansidar 与甲氟喹的人体受试者血浆中乙胺嘧啶的水平。该药物在碱性 pH 条件下用正丁基氯 - 二氯甲烷（96:4，v/v）从血浆中萃取，并在正相 HPLC 柱上通过荧光检测（激发波长 290 nm，发射波长 345 nm）进行定量。在 20 - 200 ng/ml 的浓度范围内，乙胺嘧啶几乎能从血浆中被定量萃取。使用 0.5 ml 样本时，灵敏度极限约为血浆 10 ng/ml。该方法对两种抗疟组合中的其他两种成分，即磺胺多辛和甲氟喹及其代谢物具有特异性。该测定法应用于口服 Fansidar 或 Fansidar 与甲氟喹后人体中乙胺嘧啶的药代动力学研究。