Grillot-Courvalin C, Dellagi K, Chevalier A, Brouet J C
J Immunol. 1982 Sep;129(3):1008-11.
The mode of action and the target cell of a suppressive factor continuously and spontaneously produced by a human hybrid T cell line constructed recently are reported. This factor was shown to suppress pokeweed mitogen-induced B cell differentiation when added within the first 48 hr of culture. It does not suppress B cell differentiation induced by Nocardia extracts, a relatively T-independent mitogen. The target cell for this factor seems to be a T lymphocyte in that it can be absorbed by E-rosetting cells but not by B lymphocytes or monocytes. This factor does not impair mitogen or allogeneic-induced lymphocyte proliferation and has an apparent m.w. of approximately 45,000 as estimated by Sephacryl S200 gel chromatography.
报道了最近构建的人杂交T细胞系持续自发产生的一种抑制因子的作用方式和靶细胞。该因子在培养的最初48小时内添加时，可抑制商陆有丝分裂原诱导的B细胞分化。它不抑制由诺卡氏菌提取物诱导的B细胞分化，诺卡氏菌提取物是一种相对不依赖T细胞的有丝分裂原。该因子的靶细胞似乎是T淋巴细胞，因为它可被E花环形成细胞吸收，而不能被B淋巴细胞或单核细胞吸收。该因子不损害有丝分裂原或同种异体诱导的淋巴细胞增殖，通过Sephacryl S200凝胶过滤法估计其表观分子量约为45,000。
