通过半抗原修饰抗体对细胞进行阳性和阴性选择。
Positive and negative selection of cells by hapten-modified antibodies.
作者信息
Clark N W, Parkhouse R M, Simmonds R G
出版信息
J Immunol Methods. 1982 Jun 11;51(2):167-70. doi: 10.1016/0022-1759(82)90256-3.
PMID:6980951
Abstract
A method for simultaneous positive and negative selection of cells identified by an antibody is described. It is an adaption of the panning technique using anti-benzene arsonate (Ars)-coated Petri dishes to select the Ars-antibody-coated cells. The adherent cell population is recovered in a suitable state by hapten elution with Ars-azo-tyrosine.
摘要
描述了一种对由抗体鉴定的细胞进行同时正选和负选的方法。它是一种淘选技术的改进，使用抗苯胂酸盐（Ars）包被的培养皿来选择包被有Ars抗体的细胞。通过用Ars-偶氮酪氨酸进行半抗原洗脱，以合适的状态回收贴壁细胞群体。
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