Lee D, Shochat D, Gold D V
J Natl Cancer Inst. 1982 Aug;69(2):381-5.
Human colon adenocarcinomas xenotransplanted into BALB/c nude mice were examined for the ectopic synthesis of alpha 1-proteinase inhibitor(s) (alpha 1Pi). Tumor extracts were prepared by homogenization followed by molecular sieve chromatography on Sepharose 4B. Fractions with immunoreactive alpha 1Pi were concentrated and adsorbed onto a concanavalin A-Sepharose 4B affinity column, then eluted, concentrated, and analyzed. All 4 colon tumors examined produced alpha 1Pi with full antigenic identity to normal human plasma alpha 1Pi. Electrophoretic mobilities of 3 of the tumor-derived alpha 1Pi were different from the electrophoretic mobility of the normal human plasma alpha 1Pi. Activities of the inhibitors were determined by the reaction of each tumor extract with pancreatic elastase (PE) followed by immunoelectrophoresis of the reaction mixture against both anti-human alpha 1Pi and anti-PE antisera. Two of the tumor-derived alpha 1Pi examined were active in inhibiting PE.
将人结肠腺癌异种移植到BALB/c裸鼠体内，检测其α1-蛋白酶抑制剂（α1Pi）的异位合成。通过匀浆制备肿瘤提取物，然后在Sepharose 4B上进行分子筛色谱分析。将具有免疫反应性α1Pi的组分浓缩并吸附到伴刀豆球蛋白A-Sepharose 4B亲和柱上，然后洗脱、浓缩并分析。所检测的4个结肠肿瘤均产生α1Pi，其与正常人血浆α1Pi具有完全相同的抗原性。3种肿瘤来源的α1Pi的电泳迁移率与正常人血浆α1Pi的电泳迁移率不同。通过每种肿瘤提取物与胰弹性蛋白酶（PE）反应，然后将反应混合物与抗人α1Pi和抗PE抗血清进行免疫电泳，测定抑制剂的活性。所检测的2种肿瘤来源的α1Pi在抑制PE方面具有活性。
