Johnson R G, Carty S E, Scarpa A
Biochim Biophys Acta. 1982 Jun 16;716(3):366-76. doi: 10.1016/0304-4165(82)90029-0.
The structure and function of chromaffin granules of human pheochromocytoma was extensively investigated in a highly purified granule fraction obtained from a single specimen of human pheochromocytoma tissue. Pheochromocytoma chromaffin granules were analyzed for catecholamine, ATP, enkephalin, phospholipid, cytochrome and ion content. Using a variety of techniques it was found that the membrane of these granules is highly impermeable to Na+, K+, and H+, and that the intragranular pH was maintained at 5.1 irrespective of suspending media. The presence of MgATP induces a transmembrane potential (delta psi) across the membrane of these granules which is positive inside and which corresponds to 90 mV. Both delta pH and delta psi are coupled to biogenic amine accumulation into the granules in a process which is reserpine sensitive. These properties are compared with those of chromaffin granules isolated from normal human tissue or from other animal species and are discussed in terms of possible explanation at a biochemical or subcellular level of the clinical manifestation of the pheochromocytoma.
利用从一份人嗜铬细胞瘤组织标本中获得的高度纯化的颗粒组分，对人嗜铬细胞瘤嗜铬颗粒的结构和功能进行了广泛研究。对嗜铬细胞瘤嗜铬颗粒的儿茶酚胺、ATP、脑啡肽、磷脂、细胞色素和离子含量进行了分析。使用多种技术发现，这些颗粒的膜对Na+、K+和H+具有高度不渗透性，并且无论悬浮介质如何，颗粒内的pH值都维持在5.1。MgATP的存在会在这些颗粒的膜上诱导出一种跨膜电位（δψ），其内部为正，相当于90 mV。δpH和δψ都与生物胺在颗粒中的积累相关联，这一过程对利血平敏感。将这些特性与从正常人组织或其他动物物种中分离出的嗜铬颗粒的特性进行了比较，并从生化或亚细胞水平对嗜铬细胞瘤临床表现的可能解释方面进行了讨论。
