Model P, McGill C, Mazur B, Fulford W D
Cell. 1982 Jun;29(2):329-35. doi: 10.1016/0092-8674(82)90149-0.
Two filamentous phage gene products are required for the replication of phage DNA. One of these, the gene II protein, is a site-specific endonuclease required for all phage-specific DNA synthesis. The other, the gene V protein, is a single-stranded DNA-binding protein required only for single-strand synthesis. Purified gene V protein, when added to an in vitro protein synthesizing system programmed by f1 DNA, specifically inhibits the synthesis of gene II protein. Inhibition seems to be translational, since synthesis of gene II protein from an RNA template is also inhibited by gene V protein. Gene V protein control of gene II expression can account for the regulation of the level of expression of the filamentous phage genome.
丝状噬菌体DNA的复制需要两种丝状噬菌体基因产物。其中一种是基因II蛋白，它是所有噬菌体特异性DNA合成所必需的位点特异性内切核酸酶。另一种是基因V蛋白，它是单链DNA结合蛋白，仅在单链合成时需要。纯化的基因V蛋白添加到由f1 DNA编程的体外蛋白质合成系统中时，会特异性抑制基因II蛋白的合成。这种抑制似乎是翻译水平的，因为基因V蛋白也会抑制从RNA模板合成基因II蛋白。基因V蛋白对基因II表达的控制可以解释丝状噬菌体基因组表达水平的调控。
