Lynch D H, Gurish M F, Daynes R A
Immunology. 1982 Sep;47(1):49-59.
Draining lymph nodes (DLN) from tumour immunized mice were found to contain a population of radiation resistant, Thy 1-, Ia+ antigen-presenting cells (APC) which can stimulate normal lymphocytes to differentiate into anti-tumour CTL in vitro. APC from both normal and ultraviolet (u.v.)-irradiated tumour immunized animals were compared and exhibited equivalent functional capabilities. DLN from tumour immunized u.v.-irradiated animals were also found to contain primed T cells which could differentiate into functional anti-tumour cytotoxic T lymphocytes (CTL) with the same kinetic profile as DLN cells from normal tumour immunized mice. These results indicate that u.v. irradiation does not cause a generalized depression in either the capability of APC in u.v.-irradiated mice to process and present tumour antigens, or in the ability of u.v.-exposed animals to be primed to tumour antigens. A decrease in splenic accessory cell function was detected in animals which had received 1 week of daily 30 min u.v. exposures. The decrease in splenic accessory cell function appears to be transient, however, as no decrease in this capability was found in splenic cells obtained from animals which had received daily u.v. exposures for longer periods of time (5 weeks to 5 months). Finally, the spleens of u.v.-irradiated, tumour-bearing mice were found to contain a population of cells which could impart partial protection against tumour growth in vivo. The results of the experiments presented here indicate that normal and u.v.-irradiated animals are capable of responding similarly in the afferent phases of immune responses to syngeneic tumour antigens, and suggest that u.v.-induced T-suppressor cells mediate their immunoregulatory effects at a stage late in the generation of effective anti-tumour immune responses.
在肿瘤免疫小鼠的引流淋巴结（DLN）中发现，存在一群抗辐射、Thy 1阴性、Ia阳性的抗原呈递细胞（APC），这些细胞在体外可刺激正常淋巴细胞分化为抗肿瘤细胞毒性T淋巴细胞（CTL）。对来自正常及紫外线（u.v.）照射的肿瘤免疫动物的APC进行了比较，发现它们具有同等的功能能力。还发现，来自紫外线照射的肿瘤免疫动物的DLN中含有已致敏的T细胞，这些T细胞可分化为功能性抗肿瘤细胞毒性T淋巴细胞（CTL），其动力学特征与来自正常肿瘤免疫小鼠的DLN细胞相同。这些结果表明，紫外线照射不会导致紫外线照射小鼠的APC处理和呈递肿瘤抗原的能力普遍下降，也不会导致紫外线照射动物对肿瘤抗原致敏的能力下降。在每天接受30分钟紫外线照射、持续1周的动物中，检测到脾辅助细胞功能下降。然而，脾辅助细胞功能的下降似乎是短暂的，因为在接受更长时间（5周至5个月）每日紫外线照射的动物所获得的脾细胞中，未发现这种能力下降。最后，发现紫外线照射的荷瘤小鼠的脾脏中含有一群细胞，这些细胞可在体内提供部分抗肿瘤生长的保护作用。此处呈现的实验结果表明，正常及紫外线照射的动物在对同基因肿瘤抗原的免疫反应传入阶段能够做出类似反应，并提示紫外线诱导的T抑制细胞在有效的抗肿瘤免疫反应产生后期发挥其免疫调节作用。
