Benham F J, Boccelli D, Harris H
Somatic Cell Genet. 1982 Jul;8(4):479-98. doi: 10.1007/BF01538709.
Expression of the gene locus which codes for a form of human intestinal alkaline phosphatase (ALP) has been analyzed in intraspecific somatic cell hybrids. Hybrids were constructed between D98/AH-2, a line of HeLa which ectopically synthesizes high levels of this ALP isozyme, and three different nonintestinal ALP-producing diploid lines. In chromosomally complete hybrids, expression of the ALP isozyme was initially suppressed, but on extended culture, reexpression occurred, as did limited chromosome loss. Results from extensive subcloning experiments showed that events leading to reexpression occurred at high frequency, and this ALP reexpression appeared to confer some selective advantage, direct or indirect, on the cells. In the fibroblast hybrids, reexpression of the intestinal-like ALP was always accompanied by new, high-level expression of liver/bone/kidney ALP, the product of a separate ALP gene locus. Thus expression of the one ALP locus is not excluded and, in fact, appears to be promoted by expression of the other in these cells.
已在种内体细胞杂种中分析了编码一种人肠碱性磷酸酶（ALP）形式的基因座的表达。在D98/AH-2（一种异位合成高水平这种ALP同工酶的HeLa细胞系）与三种不同的不产生肠ALP的二倍体细胞系之间构建了杂种。在染色体完整的杂种中，ALP同工酶的表达最初受到抑制，但在延长培养后，重新表达出现，同时伴有有限的染色体丢失。广泛的亚克隆实验结果表明，导致重新表达的事件高频发生，并且这种ALP重新表达似乎直接或间接地赋予细胞一些选择优势。在成纤维细胞杂种中，肠样ALP的重新表达总是伴随着肝/骨/肾ALP（另一个单独的ALP基因座的产物）新的高水平表达。因此，在这些细胞中，一个ALP基因座的表达并不被排除，事实上，似乎受到另一个基因座表达的促进。
