Louw A I, Visser L
Biochim Biophys Acta. 1978 Sep 11;512(1):163-71. doi: 10.1016/0005-2736(78)90227-4.
The synergistic effect of exogenous cobra phospholipase A2 on the hemolysis brate of guinea pig erythrocytes by highly purified snake venom cardiotoxins was investigated. In the presence of phospholipase A2 the reaction was not only faster and had a lower activation energy but followed a sigmoidal instead of a linear time course. Similar results were obtained using porcine pancreatic phospholipase A2. Significantly, addition of even a trace of cobra phospholipase A2 (approx. 0.1%, w/w) was sufficient to bring about the full synergistic effect, emphasizing the stringent purity requirements for any meaningful investigation of cardiotoxin's own action. The possibility that the action of cardiotoxin on its own may involve the stimulation of an endogenous phospholipase is discussed in the light of the results obtained with exogenous cobra enzyme.
研究了外源性眼镜蛇磷脂酶A2对高度纯化的蛇毒心脏毒素所致豚鼠红细胞溶血率的协同作用。在磷脂酶A2存在的情况下，反应不仅更快且活化能更低，而且呈现S形而非线性的时间进程。使用猪胰磷脂酶A2也获得了类似结果。值得注意的是，即使添加微量的眼镜蛇磷脂酶A2（约0.1%，w/w）也足以产生完全的协同效应，这强调了对心脏毒素自身作用进行任何有意义研究时严格的纯度要求。根据外源性眼镜蛇酶获得的结果，讨论了心脏毒素自身作用可能涉及刺激内源性磷脂酶的可能性。
