Knorr D, Bidlingmaier F, Kuhnle U
Horm Res. 1982 Jul-Aug;16(4):201-8. doi: 10.1159/000179503.
A semiautomatic capillary gas-liquid chromatographic method for the determination of urinary steroids has been developed. Trimethylsilylenol ethers were used as steroid derivatives instead of the more common methoxime trimethylsilyl ethers. The diagnosis of the various enzymatic defects causing congenital adrenal hyperplasia can be made using the characteristic pattern of urinary steroid chromatograms. Furthermore, the method presented can be used routinely to monitor therapeutic control in congenital adrenal hyperplasia. Reference data for patients of different age groups under good therapeutic control are presented.
已开发出一种用于测定尿甾体类化合物的半自动毛细管气-液色谱法。使用三甲基硅烯醇醚作为甾体衍生物，而非更为常用的甲氧肟三甲基硅醚。利用尿甾体类化合物色谱图的特征模式，可对导致先天性肾上腺皮质增生的各种酶缺陷进行诊断。此外，所提出的方法可常规用于监测先天性肾上腺皮质增生的治疗控制情况。还给出了处于良好治疗控制下不同年龄组患者的参考数据。
