麦胚凝集素对人T淋巴细胞集落形成和生长因子产生的刺激作用。

Stimulation of colony formation and growth factor production of human T lymphocytes by wheat germ lectin.

作者信息

Ulmer A J, Scholz W, Flad H D

出版信息

Immunology. 1982 Nov;47(3):551-6.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1555539/

Abstract

Wheat germ lectin (WGL) induced proliferation of T-lymphocyte colony-forming units (TL-CFU) from human peripheral blood mononuclear cells (PBMC) in a one stage agar culture needing higher cell numbers than phytohaemagglutinin (PHA). However, WGL preactivated TL-CFU in a first stage liquid culture for the growth of PHA-dependent T-lymphocyte colonies in a subsequent agar culture. These results suggested that WGL and PHA have biological activities in common. WGL and PHA both induced the expression of Interleukin 2 (IL-2) receptors by PBMC to become responsive to IL-2, although production of IL-2 by WGL-stimulated PBMC was low compared with PHA-stimulated PBMC. This might account for the low proliferative response of PBMC to WGL.

摘要

小麦胚凝集素（WGL）在单阶段琼脂培养中可诱导人外周血单个核细胞（PBMC）的T淋巴细胞集落形成单位（TL-CFU）增殖，与植物血凝素（PHA）相比，该培养需要更高的细胞数量。然而，WGL在第一阶段液体培养中预激活TL-CFU，以便在随后的琼脂培养中生长依赖PHA的T淋巴细胞集落。这些结果表明WGL和PHA具有共同的生物学活性。WGL和PHA均可诱导PBMC表达白细胞介素2（IL-2）受体，使其对IL-2产生反应，尽管与PHA刺激的PBMC相比，WGL刺激的PBMC产生的IL-2量较低。这可能解释了PBMC对WGL的增殖反应较低的原因。

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Identification of a tumor-specific determinant on neoplastic cell surfaces.肿瘤细胞表面肿瘤特异性决定簇的鉴定。

Proc Natl Acad Sci U S A. 1967 Feb;57(2):359-66. doi: 10.1073/pnas.57.2.359.

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REACTIONS OF NORMAL AND TUMOR CELL SURFACES TO ENZYMES. I. WHEAT-GERM LIPASE AND ASSOCIATED MUCOPOLYSACCHARIDES.正常细胞与肿瘤细胞表面对酶的反应。I. 小麦胚芽脂肪酶及相关粘多糖

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The activation of blast transformation and DNA synthesis in human peripheral blood lymphocytes by wheat germ agglutinin.麦胚凝集素对人外周血淋巴细胞的胚细胞转化和DNA合成的激活作用。

J Immunol. 1980 Aug;125(2):814-9.

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T-cell growth factor.T细胞生长因子。

Immunol Rev. 1980;51:337-57. doi: 10.1111/j.1600-065x.1980.tb00327.x.

5

Interaction of human lymphocyte subpopulations with interleukin 2 (II-2).人类淋巴细胞亚群与白细胞介素2（IL-2）的相互作用。

Eur J Cell Biol. 1981 Aug;25(1):16-8.

6

Inhibition of human lymphocyte proliferation by the nonmitogenic lectin wheat germ agglutinin.非促有丝分裂凝集素麦胚凝集素对人淋巴细胞增殖的抑制作用。

J Immunol. 1980 Jun;124(6):2979-87.

7

The purification, composition and specificity of wheat-germ agglutinin.小麦胚芽凝集素的纯化、组成及特异性

Biochem J. 1973 Jan;131(1):155-62. doi: 10.1042/bj1310155.

8

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Exp Cell Res. 1973 Jan;76(1):143-51. doi: 10.1016/0014-4827(73)90429-1.

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Int Arch Allergy Appl Immunol. 1973;45(3):331-40. doi: 10.1159/000231051.

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