少数衣壳蛋白亚基对苜蓿花叶病毒RNA种类碱基配对结构的影响

Influence of a few coat protein subunits on the base-paired structure of the RNA species of alfalfa mosaic virus.

作者信息

Srinivasan S, Jaspars E M

出版信息

Biochim Biophys Acta. 1978 Aug 23;520(1):237-41. doi: 10.1016/0005-2787(78)90025-4.

Abstract

Differentiated thermal melting profiles were made of all the three RNA species (RNAs 1, 2 and 3), constituting the genome of alfalfa mosaic virus and of the subgenomic coat protein messenger RNA (RNA 4) of this virus. Whereas all profiles showed multiphasicity the profile of RNA 4 was most clearly subdivided showing three clear transitions with tm values of 25.5, 35.5 and 53 degrees C. The first transition disappeared upon addition of 6 coat protein molecules per molecule of RNA 4. The effect of coat protein on the melting profiles of the genome RNAs was much less clear.

摘要

对构成苜蓿花叶病毒基因组的所有三种RNA种类（RNA 1、2和3）以及该病毒的亚基因组外壳蛋白信使RNA（RNA 4）进行了差示热解链分析。虽然所有的分析图谱都显示出多相性，但RNA 4的图谱划分最为清晰，显示出三个明显的转变，解链温度（tm）值分别为25.5、35.5和53摄氏度。每分子RNA 4添加6个外壳蛋白分子后，第一个转变消失。外壳蛋白对基因组RNA解链图谱的影响则不太明显。