人肝脏中胆汁盐的酶促硫酸化作用。
Enzymatic sulphation of bile salts in human liver.
作者信息
Lööf L, Wengle B
出版信息
Biochim Biophys Acta. 1978 Sep 28;530(3):451-60. doi: 10.1016/0005-2760(78)90165-0.
An enzyme catalyzing the transfer of the sulphate group from 3' -phosphoadenosine-5' -phosphosulphate to lithocholate and glycolithocholate is identified in the cytosol of human liver. The rate of sulphation was greatest with unconjugates lithocolate. Km values for lithocholate and glycolithocholate were 2 . 10(-6) and 3.3 . 10(-6) M, respectively. No enzyme activity was found in human kidney cytosol. A simple method for quantitative assay of the enzyme in percutaneous liver biopsy specimens is described.
在人肝脏细胞溶质中鉴定出一种能催化硫酸基团从3'-磷酸腺苷-5'-磷酸硫酸转移至石胆酸和甘氨石胆酸的酶。硫酸化速率在未结合的石胆酸时最高。石胆酸和甘氨石胆酸的米氏常数分别为2×10⁻⁶和3.3×10⁻⁶ M。在人肾脏细胞溶质中未发现酶活性。描述了一种用于经皮肝活检标本中该酶定量测定的简单方法。
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