Kabanov V A, Mustafaev M I, Belova V V, Evdakov V P
Biofizika. 1978 Sep-Oct;23(5):789-95.
It has been shown by high-velocity sedimentation that BSA forms soluble complexes with polyacrylic acid (PAA) and sodium polystyrene sulfonate (PSSNa) with neutral water solutions. Factors responsible for polyanion binding of similarly charged BSA molecules are analysed. It has been found that occupation of PAA and PSSNa chains with BSA globules proceeds steadily. BSA-PAA and BSA-PSSNa complexes are compared with the earlier described products of BSA sorption on poly-4-vinyl-N-ethylpyridine bromide, where the occupation of polycations with protein globules proceeds unevenly. Reasons for the existence of two types of soluble BSA-polyelectrolyte complexes are described; probable structural model of BSA complex with polyacids and polyanions is suggested.
高速沉降实验表明，在中性水溶液中，牛血清白蛋白（BSA）与聚丙烯酸（PAA）和聚苯乙烯磺酸钠（PSSNa）形成可溶性复合物。分析了带相同电荷的BSA分子与聚阴离子结合的相关因素。研究发现，BSA球蛋白在PAA和PSSNa链上的占据过程是稳定的。将BSA-PAA和BSA-PSSNa复合物与先前描述的BSA在聚-4-乙烯基-N-乙基吡啶溴化物上的吸附产物进行了比较，在后者中，蛋白质球蛋白在聚阳离子上的占据过程是不均匀的。阐述了两种可溶性BSA-聚电解质复合物存在的原因；提出了BSA与聚酸和聚阴离子复合物可能的结构模型。
