Orlando J A, Pisani T
Can J Biochem. 1982 Sep;60(9):882-9. doi: 10.1139/o82-113.
The light-dependent transhydrogenase system of Rhodopseudomonas spheroides which consists of a peripheral protein factor and a membrane-bound component contains essential sulfhydryl groups that are sensitive to p-hydroxymercuribenzoic acid (PMB). There are two types of sulfhydryl groups required for light-dependent (LD) transhydrogenation. One type is associated with the membrane-bound component and participates in or influences the binding of one of the substrates, NADH. A second type is associated with the peripheral protein factor and is not involved with the binding of the substrates. These sulfhydryl groups are masked or buried in the protein and are only exposed upon treatment of the peripheral protein factor with urea or trypsin. The peripheral protein factor may be an integral part of the transhydrogenase complex or may be required for the energization process. This factor appears to play an important role in the activation and control of LD-transhydrogenation.
球形红假单胞菌的光依赖型转氢酶系统由一个外周蛋白因子和一个膜结合组分组成，该系统含有对对羟基汞苯甲酸（PMB）敏感的必需巯基。光依赖型（LD）转氢作用需要两种类型的巯基。一种类型与膜结合组分相关，参与或影响底物之一NADH的结合。第二种类型与外周蛋白因子相关，不参与底物的结合。这些巯基被掩盖或埋藏在蛋白质中，只有在用尿素或胰蛋白酶处理外周蛋白因子后才会暴露出来。外周蛋白因子可能是转氢酶复合物的一个组成部分，或者是能量化过程所必需的。这个因子似乎在LD转氢作用的激活和控制中起重要作用。
