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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Okazaki T, Ishihara H, Shukuya R

Comp Biochem Physiol B. 1982;73(2):309-12. doi: 10.1016/0305-0491(82)90289-9.

The circulating erythrocytes of Rana catesbeiana tadpoles at varying metamorphic stages were studied by the phthalate-microcapillary technique of Danon & Marikovsky (1964 J. Lab. Clin. Med. 64, 668-674) and by the method of Murphy (1973 J. Lab. Clin. Med. 82, 334-341), which are used for the separation of erythrocytes according to the density. 2. The erythrocytes of tadpoles were different in the mean density from those of adult frogs. 3. At the metamorphic climax, aged erythrocytes having the tadpole hemoglobin coexisted with young erythrocytes having the frog hemoglobin in the circulating blood. 4. The frog hemoglobin was preferentially synthesized during metamorphosis. 5. All the results suggested that the transition of hemoglobin during metamorphosis was associated with the replacement of erythrocyte population.

采用达农和马里科夫斯基（1964年，《实验与临床检验医学杂志》第64卷，第668 - 674页）的邻苯二甲酸微毛细管技术以及墨菲（1973年，《实验与临床检验医学杂志》第82卷，第334 - 341页）的方法，对处于不同变态阶段的牛蛙蝌蚪的循环红细胞进行了研究，这两种方法用于根据密度分离红细胞。2. 蝌蚪的红细胞平均密度与成年青蛙的不同。3. 在变态高峰期，含有蝌蚪血红蛋白的老化红细胞与含有青蛙血红蛋白的年轻红细胞共存于循环血液中。4. 青蛙血红蛋白在变态过程中优先合成。5. 所有结果表明，变态过程中血红蛋白的转变与红细胞群体的更替有关。

Okazaki T, Ishihara H, Shukuya R

Comp Biochem Physiol B. 1982;73(2):309-12. doi: 10.1016/0305-0491(82)90289-9.

The circulating erythrocytes of Rana catesbeiana tadpoles at varying metamorphic stages were studied by the phthalate-microcapillary technique of Danon & Marikovsky (1964 J. Lab. Clin. Med. 64, 668-674) and by the method of Murphy (1973 J. Lab. Clin. Med. 82, 334-341), which are used for the separation of erythrocytes according to the density. 2. The erythrocytes of tadpoles were different in the mean density from those of adult frogs. 3. At the metamorphic climax, aged erythrocytes having the tadpole hemoglobin coexisted with young erythrocytes having the frog hemoglobin in the circulating blood. 4. The frog hemoglobin was preferentially synthesized during metamorphosis. 5. All the results suggested that the transition of hemoglobin during metamorphosis was associated with the replacement of erythrocyte population.

采用达农和马里科夫斯基（1964年，《实验与临床检验医学杂志》第64卷，第668 - 674页）的邻苯二甲酸微毛细管技术以及墨菲（1973年，《实验与临床检验医学杂志》第82卷，第334 - 341页）的方法，对处于不同变态阶段的牛蛙蝌蚪的循环红细胞进行了研究，这两种方法用于根据密度分离红细胞。2. 蝌蚪的红细胞平均密度与成年青蛙的不同。3. 在变态高峰期，含有蝌蚪血红蛋白的老化红细胞与含有青蛙血红蛋白的年轻红细胞共存于循环血液中。4. 青蛙血红蛋白在变态过程中优先合成。5. 所有结果表明，变态过程中血红蛋白的转变与红细胞群体的更替有关。