Suzuki S, Fujikura K
Department of Comparative Endocrinology, Gunma University, Maebashi, Japan.
Gen Comp Endocrinol. 1994 Apr;94(1):72-7. doi: 10.1006/gcen.1994.1061.
Using purified thyroglobulin (TG) of Rana catesbeiana and its antisera, a specific double-antibody radioimmunoassay (RIA) was developed. Serum TG content was measured in tadpoles, froglets, and adult frogs by RIA. Serum TG increased during pre- and prometamorphosis and reached a peak of 476.9 +/- 29.1 ng/ml at the climax. Thereafter the amount of circulating TG decreased slowly toward the end of metamorphosis, and it was at the lowest level (154.1 +/- 18.4 ng/ml) in froglets. Serum TG in adult frogs was 267.3 +/- 31.7 ng/ml. The serum TG in tadpoles and adult frogs was identified by immunoblotting as only one component corresponding to the purified TG. The circulating TG level was much higher than that in mammalian species and chickens reported so far. These results seem to show that the release of TG is a preferential function in the bullfrog thyroid gland.
利用牛蛙纯化甲状腺球蛋白（TG）及其抗血清，建立了一种特异性双抗体放射免疫分析法（RIA）。采用RIA法测定蝌蚪、幼蛙和成蛙血清中的TG含量。血清TG在前变态期和前变态高峰期升高，在变态高峰期达到476.9±29.1 ng/ml的峰值。此后，循环TG的量在变态末期缓慢下降，在幼蛙中处于最低水平（154.1±18.4 ng/ml）。成蛙血清TG为267.3±31.7 ng/ml。通过免疫印迹法鉴定，蝌蚪和成蛙血清中的TG仅为一种与纯化TG相对应的成分。循环TG水平远高于迄今报道的哺乳动物和鸡的水平。这些结果似乎表明，TG的释放是牛蛙甲状腺的一个优先功能。
