del Castillo J, Specht P, Auerbach A
J Neurosci Res. 1982;8(1):35-48. doi: 10.1002/jnr.490080107.
Two techniques are described that are designed to elicit long and constant postsynaptic depolarizations or agonist-induced currents by the electrophoretic application of agonists to endplate membranes. In the first technique, which can be described as a "pharmacologic voltage clamp," the current flowing through the agonist pipette is controlled by the potential across the endplate membrane. Sustained depolarizations of constant amplitude can be obtained in this manner, while the agonist current needed to produce and maintain the response is recorded. The second technique uses a signal proportional to the current flowing across a voltage-clamped endplate membrane to control the delivery of agonist. This allows control of the intensity of the agonist-induced current. The possible application of these techniques in studying receptor desensitization and obtaining samples of post-synaptic current noise for spectral analysis is discussed.
本文描述了两种技术，其设计目的是通过向终板膜电泳施加激动剂来引发长时间且持续的突触后去极化或激动剂诱导的电流。在第一种技术中，可被描述为“药理学电压钳”，流经激动剂移液管的电流由终板膜上的电位控制。通过这种方式可以获得恒定幅度的持续去极化，同时记录产生和维持该反应所需的激动剂电流。第二种技术使用与流经电压钳制终板膜的电流成比例的信号来控制激动剂的递送。这允许控制激动剂诱导电流的强度。讨论了这些技术在研究受体脱敏以及获取用于频谱分析的突触后电流噪声样本方面的可能应用。
