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Hpa I甲基化酶的纯化及性质

Purification and properties of the Hpa I methylase.

作者信息

Yoo O J, Dwyer-Hallquist P, Agarwal K L

出版信息

Nucleic Acids Res. 1982 Oct 25;10(20):6511-9. doi: 10.1093/nar/10.20.6511.

DOI:10.1093/nar/10.20.6511
PMID:6983681
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326940/
Abstract

The purification and catalytic properties of the homogeneous Hpa I methylase is described. The enzyme exists as a single polypeptide chain with a molecular weight of 37,000 +/- 2,000 was shown by sedimentation equilibrium and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The Hpa I methylase transfers methyl groups of S-adenosylmethionine to adenine present in the recognition sequence d(G-T-T-A-A*-C), A* is the N6 methyl adenosine. An average of 2.1 methyl groups per recognition site are transferred by the Hpa I methylase.

摘要

本文描述了同源Hpa I甲基化酶的纯化及其催化特性。沉降平衡实验和十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳结果表明，该酶以单条多肽链的形式存在，分子量为37,000±2,000。Hpa I甲基化酶将S-腺苷甲硫氨酸的甲基转移至识别序列d(G-T-T-A-A*-C)中的腺嘌呤上，其中A*为N6-甲基腺苷。每个识别位点平均有2.1个甲基被Hpa I甲基化酶转移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d69/326940/89c7bfe851d1/nar00389-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d69/326940/d47671e370d8/nar00389-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d69/326940/89c7bfe851d1/nar00389-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d69/326940/d47671e370d8/nar00389-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d69/326940/89c7bfe851d1/nar00389-0291-a.jpg

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1
Purification and properties of the Hpa I methylase.Hpa I甲基化酶的纯化及性质
Nucleic Acids Res. 1982 Oct 25;10(20):6511-9. doi: 10.1093/nar/10.20.6511.
2
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引用本文的文献

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The effect of site specific methylation on restriction endonuclease cleavage (update).位点特异性甲基化对限制性内切酶切割的影响（更新）。

本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.一种来自流感嗜血杆菌的限制性内切酶。I. 纯化及一般特性。
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CXII. Total synthesis of the structural gene for an alanine transfer RNA from yeast. Enzymic joining of the chemically synthesized polydeoxynucleotides to form the DNA duplex representing nucleotide sequence 1 to 20.CXXII. 酵母丙氨酸转移核糖核酸结构基因的全合成。化学合成的多脱氧核苷酸的酶促连接以形成代表核苷酸序列1至20的DNA双链体。
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