血清和表皮生长因子可使静止的BSC-1上皮细胞短暂去极化。
Serum and epidermal growth factor transiently depolarize quiescent BSC-1 epithelial cells.
作者信息
Rothenberg P, Reuss L, Glaser L
出版信息
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7783-7. doi: 10.1073/pnas.79.24.7783.
Abstract
Continuous intracellular recording of membrane potential with microelectrodes in BSC-1 epithelial cells has been used to study the early ionic effects of the interaction of mitogens with cell surface receptors. Initial results show that (i) there is no significant difference in membrane potential between growing and quiescent cells, (ii) addition of epidermal growth factor or serum to quiescent BSC-1 cells induces a brief and transient depolarization, (iii) the mitogenic response of BSC-1 cells to epidermal growth factor and the transient depolarization show similar concentration dependences, and (iv) serum addition to quiescent BSC-1 cells induces a sustained increase in Na+ influx that is electroneutral and amiloride sensitive. Intracellular pH changes may be a primary event triggering the response of quiescent cells to mitogenic polypeptides.
摘要
利用微电极对BSC - 1上皮细胞的膜电位进行连续细胞内记录，以研究促细胞分裂剂与细胞表面受体相互作用的早期离子效应。初步结果表明：（i）生长细胞和静止细胞的膜电位无显著差异；（ii）向静止的BSC - 1细胞中添加表皮生长因子或血清会诱导短暂的瞬时去极化；（iii）BSC - 1细胞对表皮生长因子的促有丝分裂反应和瞬时去极化表现出相似的浓度依赖性；（iv）向静止的BSC - 1细胞中添加血清会诱导Na⁺内流持续增加，且该过程是电中性的且对氨氯地平敏感。细胞内pH变化可能是触发静止细胞对促有丝分裂多肽反应的主要事件。
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