Grafe P, Rimpel J, Reddy M M, ten Bruggencate G
Neuroscience. 1982;7(12):3213-20. doi: 10.1016/0306-4522(82)90243-3.
A post-tetanic membrane hyperpolarization following repetitive neuronal activity is a commonly observed phenomenon in the isolated frog spinal cord as well as in neurons of other nervous tissues. We have now used double-barrelled Na+- and K+-ion-sensitive microelectrodes to measure the intracellular Na+- and K+-concentrations and also the extracellular K+-concentration of lumbar spinal motoneurons during and after repetitive stimulation of a dorsal root. The results show that the post-tetanic membrane hyperpolarization occurred at a time when the intracellular [Na+] reached its maximal value, intracellular [K+] had its lowest level and extracellular [K+] was still elevated. The hyperpolarization was blocked by ouabain and reduced by Li+. These data support the previous suggestion that an electrogenic Na+/K+ pump mode may be the mechanism underlying the post-tetanic membrane hyperpolarization.
在重复神经元活动后出现的强直后膜超极化，是在离体蛙脊髓以及其他神经组织的神经元中普遍观察到的现象。我们现在使用双管钠钾离子敏感微电极，来测量在重复刺激背根期间及之后腰段脊髓运动神经元的细胞内钠钾浓度以及细胞外钾浓度。结果显示，强直后膜超极化发生在细胞内[Na⁺]达到最大值、细胞内[K⁺]处于最低水平且细胞外[K⁺]仍升高之时。超极化被哇巴因阻断，被Li⁺减弱。这些数据支持了之前的推测，即电生性钠钾泵模式可能是强直后膜超极化的潜在机制。
