使用中性载体钠离子选择性微电极进行细胞内钠活性测量。
Intracellular measurement of Na activity using neutral carrier Na ion-selective microelectrode.
作者信息
Kajino K, Fujimoto M
出版信息
Jpn J Physiol. 1982;32(6):997-1001. doi: 10.2170/jjphysiol.32.997.
PMID:6984868
Abstract
To measure intracellular Na+ activities, a double-barreled Na ion-selective microelectrode was constructed with a neutral carrier Na+ ligand (ETH 227). The slope constant and detection limit were 56 mV and 3-5 mM, respectively, the selectivity of Na+/K+ being 40-50. In the bullfrog, the cell Na+ was 14.4 mEq/liter for the proximal tubule and 12.6 mEq/liter for the sartorius muscle.
摘要
为了测量细胞内钠离子活性，使用中性载体钠离子配体（ETH 227）构建了双管钠离子选择性微电极。斜率常数和检测限分别为56 mV和3 - 5 mM，钠离子/钾离子选择性为40 - 50。在牛蛙中，近端小管的细胞内钠离子浓度为14.4 mEq/升，缝匠肌的为12.6 mEq/升。
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