Antoine J L, Couturier J, Dutrillaux B
Ann Genet. 1982;25(4):223-5.
A study is reported on the location of X chromosomes in human female lymphocytes and fibroblasts at mitosis. By the use of BrdU, incorporated during either early or late S-phase, the late and the early replicating Xs were identified, so that it could be determined which was more peripheral in the metaphase plate. In lymphocytes, when BrdU is incorporated during late S-phase, the late-replicating X, which is the less condensed, is the more peripheral. On the contrary, when BrdU is incorporated during early S-phase, the early replicating X, which is the less condensed, is the more peripheral. On the other hand, in fibroblasts grown on coverslips, the late-replicating X is always peripheral, regardless of the time of BrdU treatment. These results confirm the peripheral location of the late-replicating X and demonstrate the presence of artifacts, depending on chromosome condensation, occurring during the procedures of slide preparation.
本文报道了一项关于有丝分裂期人类女性淋巴细胞和成纤维细胞中X染色体定位的研究。通过使用在S期早期或晚期掺入的5-溴脱氧尿嘧啶核苷（BrdU），鉴定出晚期和早期复制的X染色体，从而能够确定在中期板中哪条染色体更靠近外周。在淋巴细胞中，当在S期晚期掺入BrdU时，复制较晚、凝聚程度较低的X染色体更靠近外周。相反，当在S期早期掺入BrdU时，复制较早、凝聚程度较低的X染色体更靠近外周。另一方面，在盖玻片上生长的成纤维细胞中，无论BrdU处理的时间如何，复制较晚的X染色体总是位于外周。这些结果证实了复制较晚的X染色体的外周定位，并证明了在玻片制备过程中，根据染色体凝聚情况会出现假象。
