Jaczewska A, Pastuszewska B, Bartik M, Kowalczyk J
Acta Physiol Pol. 1982 Jul-Aug;33(4):361-8.
Pancreatic secretion in rats was assessed by measuring the amounts of p-aminobenzoic acid (PABH) excreted in urine after oral administration of Ac-L-Tyr-PAB, and by determination of enzyme activity in pancreas and faeces. 3 groups of 7 rats were kept on diets with casein as the main source of nitrogen without (control K0) or with two levels of trypsin inhibitor (K1 and K2). Two other groups were fed diets with 40 and 80% of casein substituted by raw soya bean protein and having trypsin inhibitor contents equivalent to those in diets K1 and K2. Urinary excretion of PABH ranged from 99 to 105% of intake and were not different between control and experimental rats. The weight of pancreas and pancreatic activities of trypsin and chymotrypsin in all experimental rats were higher than in controls. The activity of chymotrypsin in the faeces of rats of groups K1 and K2 was greater than in K0 while in groups S1 and S2 it was about five times that in groups K1 and K2. Generally, it was greater on diets with more trypsin inhibitor. The activity of trypsin in the faeces of rats K0, K1 and S1 was several times less than in K2 and S2. It has been concluded that measurement of trypsin and chymotrypsin in faeces allows to estimate differences in the secretion of pancreatic enzymes.
通过测量口服乙酰-L-酪氨酸-对氨基苯甲酸（Ac-L-Tyr-PAB）后尿中对氨基苯甲酸（PABH）的排泄量，以及测定胰腺和粪便中的酶活性，来评估大鼠的胰腺分泌情况。将3组每组7只大鼠分别喂以以酪蛋白作为主要氮源的饲料，一组不添加胰蛋白酶抑制剂（对照K0），另外两组添加两种水平的胰蛋白酶抑制剂（K1和K2）。另外两组喂以用生大豆蛋白替代40%和80%酪蛋白且胰蛋白酶抑制剂含量与饲料K1和K2相当的饲料。PABH的尿排泄量为摄入量的99%至105%，对照大鼠和实验大鼠之间无差异。所有实验大鼠的胰腺重量以及胰蛋白酶和糜蛋白酶的胰腺活性均高于对照大鼠。K1组和K2组大鼠粪便中糜蛋白酶的活性高于K0组，而S1组和S2组大鼠粪便中糜蛋白酶的活性约为K1组和K2组的五倍。一般来说，胰蛋白酶抑制剂含量越高的饲料，糜蛋白酶活性越高。K0组、K1组和S1组大鼠粪便中胰蛋白酶的活性比K2组和S2组低几倍。得出的结论是，通过测量粪便中的胰蛋白酶和糜蛋白酶可以估计胰腺酶分泌的差异。
