Meagher R C, Spivak J L, Sensenbrenner L L, Sharkis S J
Stem Cells (1981). 1982 Nov-Dec;2(6):321-34.
Medium conditioned by mouse thymic cells cultured for 7 days in the presence of concanavalin A (Con A) and 5% human serum enhanced erythroid colony formation by CFU-E but not BFU-E. Medium conditioned by thymic cells for less than 7 days or more than 7 days inhibited erythroid colony formation. Enhancement of erythroid colony formation occurred at both suboptimal and saturating concentrations of erythropoietin. The thymus-conditioned medium also stimulated the growth of granulocyte/macrophage colonies by itself and in the presence of a saturating concentration of WEHI-3 conditioned medium. The conditioned medium contained neither burst-promoting activity nor erythropoietin. The erythroid colony-enhancing activity in the thymus-conditioned medium was heat stable, bound to immobilized Con A, lacked protease activity and migrated with an approximate molecular weight of 68,000 on gel filtration.
在伴刀豆球蛋白A（Con A）和5%人血清存在的情况下培养7天的小鼠胸腺细胞所产生的条件培养基增强了CFU-E的红系集落形成，但未增强BFU-E的红系集落形成。胸腺细胞培养少于7天或多于7天所产生的条件培养基抑制红系集落形成。在促红细胞生成素的次优浓度和饱和浓度下均出现了红系集落形成的增强。胸腺条件培养基自身以及在存在饱和浓度的WEHI-3条件培养基的情况下也刺激了粒细胞/巨噬细胞集落的生长。该条件培养基既不含有爆式促进活性，也不含有促红细胞生成素。胸腺条件培养基中的红系集落增强活性是热稳定的，与固定化的Con A结合，缺乏蛋白酶活性，并且在凝胶过滤中以约68,000的分子量迁移。
