Vánky F, Klein E
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Cancer Immunol Immunother. 1982;14(2):73-7. doi: 10.1007/BF00200170.
T-cell cultures derived from the blood of 14 patients with solid tumors were propagated with T-cell growth factor (TCGF). The cultures were initiated from lymphocytes exposed to autologous tumor-biopsy cells. TCGF was added either immediately or 3-10 days later. In the former culture type the cell yield on day 7 was considerably higher. The cytotoxic potential of the cultured cells was assayed on two occasions, between days 7 and 10 and between weeks 5 and 8. Cells of all but two cultures had the potential to lyse autologous tumor-biopsy cells. On the population level, cytotoxicity was specific for autologous tumor in those cultures that were driven to growth with TCGF after the 3rd day. These lymphocytes did not lyse allogeneic tumor-biopsy cells. In contrast, all five cultures initiated in the presence of TCGF exhibited a broader cytotoxic potential, i.e., in addition to the stimulator autologous-tumor cells, they also lysed other targets. Another difference between the two culture types was their behavior toward K562. Tested on the 7th day they all lysed K562; however, this function declined in strength or disappeared later in the cultures exposed to TCGF after the 3rd day. Reexposure of the lymphocytes to autologous tumor-biopsy cells after 2 weeks of culture period, but not on the 7th day, induced DNA synthesis. This secondary response was specific inasmuch as allogeneic tumor cells had little or no effect. One of the autotumor restimulated cultures was tested for cytotoxic potential. It increased against the autologous but not against other tumors or K562 cells.
从14例实体瘤患者血液中获取的T细胞培养物，用T细胞生长因子（TCGF）进行增殖培养。培养物由接触自体肿瘤活检细胞的淋巴细胞起始。TCGF要么立即添加，要么在3 - 10天后添加。在前一种培养类型中，第7天的细胞产量显著更高。在第7至10天以及第5至8周期间，对培养细胞的细胞毒性潜力进行了两次检测。除了两个培养物外，所有培养物的细胞都有裂解自体肿瘤活检细胞的潜力。在群体水平上，对于那些在第3天后用TCGF驱动生长的培养物，细胞毒性对自体肿瘤具有特异性。这些淋巴细胞不裂解同种异体肿瘤活检细胞。相比之下，在TCGF存在下起始的所有五个培养物都表现出更广泛的细胞毒性潜力，即除了刺激物自体肿瘤细胞外，它们还能裂解其他靶细胞。两种培养类型的另一个差异在于它们对K562的反应。在第7天检测时，它们都能裂解K562；然而，在第3天后接触TCGF的培养物中，这种功能在后期强度下降或消失。培养2周后，而非在第7天，将淋巴细胞重新暴露于自体肿瘤活检细胞可诱导DNA合成。这种二次反应具有特异性，因为同种异体肿瘤细胞几乎没有影响。对其中一个自体肿瘤再刺激的培养物进行了细胞毒性潜力检测。它对自体肿瘤的细胞毒性增加，但对其他肿瘤或K562细胞没有作用。
