神经元特异性烯醇化酶在组织培养中生长的小鼠脊髓神经元中的定位。

Localization of neuron-specific enolase in mouse spinal neurons grown in tissue culture.

作者信息

Schmechel D E, Brightman M W, Barker J L

出版信息

Brain Res. 1980 Jan 13;181(2):391-400. doi: 10.1016/0006-8993(80)90621-6.

Abstract

Neuron-specific enolase (NSE) is an isoenzyme of the glycolytic enzyme enolase (EC 4.2.1.11) which also has a muscle and liver isoenzyme. Previous work has shown NSE to be specifically localized to neurons and neuroendocrine cells, but the application of NSE as a marker for cell cultures has not been investigated. Primary culture of central nervous system tissue derived from mice have been used to study optimal fixation procedures. The results show that NSE can serve as a useful alternative to non-specific histochemical strains or strictly morphologic criteria for identifying nerve cells.

摘要

神经元特异性烯醇化酶（NSE）是糖酵解酶烯醇化酶（EC 4.2.1.11）的一种同工酶，烯醇化酶还有肌肉和肝脏同工酶。以往的研究表明，NSE特异性定位于神经元和神经内分泌细胞，但尚未研究将NSE用作细胞培养标志物的应用。源自小鼠的中枢神经系统组织的原代培养已用于研究最佳固定程序。结果表明，NSE可作为非特异性组织化学染色或严格形态学标准的有用替代物，用于识别神经细胞。