Bertoli A, De Pirro R, Fusco A, Greco A V, Magnatta R, Lauro R
J Clin Endocrinol Metab. 1980 Feb;50(2):246-50. doi: 10.1210/jcem-50-2-246.
Specific binding of [125I]insulin to circulating monocytes and erythrocytes from nine normal menstruating women and nine normal men was determined during a 28-day period (one sample every 7 days). In women, insulin binding was higher to both monocytes (P less than 0.001) and erythrocytes (P less than 0.02) in the follicular phase than in the luteal phase. In men, insulin binding to monocytes was similar to the follicular phase values for women; however, insulin binding to erythrocytes from men showed higher values than insulin binding to erythrocytes from women in both the follicular (P less than 0.001) and luteal (P less than 0.001) phases. These differences were due primarily to changes in receptor concentration rather than receptor affinity. An inverse relationship was found between insulin binding to monocytes and levels of 17 beta-estradiol, progesterone, and 17 alpha-hydroxyprogesterone; this relationship was not observed in insulin binding to erythrocytes. The present data, therefore, suggest that sex hormones may play a role in the control of insulin receptors. Furthermore, it appears that other factors exist during the follicular phase that lower insulin binding to erythrocyte insulin receptors. If insulin receptors on circulating cells reflect the behavior of the main insulin target tissues, the present data might in part explain the reduction in glucose tolerance reported by various authors in the second half of the menstrual cycle.
测定了9名正常月经周期女性和9名正常男性循环单核细胞和红细胞对[125I]胰岛素的特异性结合，为期28天（每7天取一次样本）。在女性中，卵泡期单核细胞（P<0.001）和红细胞（P<0.02）的胰岛素结合均高于黄体期。在男性中，单核细胞的胰岛素结合与女性卵泡期的值相似；然而，在卵泡期（P<0.001）和黄体期（P<0.001），男性红细胞的胰岛素结合值均高于女性红细胞的胰岛素结合值。这些差异主要是由于受体浓度的变化而非受体亲和力的变化。发现胰岛素与单核细胞的结合与17β-雌二醇、孕酮和17α-羟孕酮水平呈负相关；在胰岛素与红细胞的结合中未观察到这种关系。因此，目前的数据表明性激素可能在胰岛素受体的调控中起作用。此外，似乎在卵泡期存在其他因素降低了胰岛素与红细胞胰岛素受体的结合。如果循环细胞上的胰岛素受体反映了主要胰岛素靶组织的行为，那么目前的数据可能部分解释了不同作者报道的月经周期后半期葡萄糖耐量降低的原因。
