Wise P M, Ratner A
Neuroendocrinology. 1980;30(1):15-9. doi: 10.1159/000122968.
Resting plasma concentrations of LH, FSH, estradiol, and progesterone and medial basal hypothalamic concentrations of LHRH (MBH-LHRH) were measured by RIA in 8- to 12-month-old female rats which had begun to exhibit constant estrous (CE) or prolonged diestrous (PD) vaginal smear patterns and compared to young cycling rats on proestrus, estrus, or diestrus. In addition, we examined the effect of ovariectomy on these hormonal profiles. Old CE rats have normal plasma LH, FSH and progesterone concentrations, but exhibit elevated estradiol levels and decreased MBH-LHRH concentrations compared to young cycling rats on the day of estrus. Ovariectomy results in an attenuated rise in plasma LH and FSH and a much lesser decrease in MBH-LHRH when compared to young rats, despite comparable steroid changes. Old PD rats have normal LH and FSH levels,but have elevated estradiol and progesterone concentrations and decreased MBH-LHRH levels when compared to young rats on the day of diestrus. Ovariectomy causes a normal decrease in MBH-LHRH; however, the increased gonadotropin levels are significantly less than seen in young controls.
通过放射免疫分析法（RIA）测定了8至12月龄雌性大鼠的促黄体生成素（LH）、促卵泡生成素（FSH）、雌二醇和孕酮的静息血浆浓度，以及下丘脑内侧基底部促性腺激素释放激素（MBH-LHRH）的浓度。这些大鼠已开始表现出持续发情（CE）或延长动情间期（PD）的阴道涂片模式，并与处于发情前期、发情期或动情间期的年轻周期性发情大鼠进行了比较。此外，我们研究了卵巢切除术对这些激素水平的影响。与处于发情期的年轻周期性发情大鼠相比，老龄CE大鼠的血浆LH、FSH和孕酮浓度正常，但雌二醇水平升高，MBH-LHRH浓度降低。与年轻大鼠相比，卵巢切除术导致老龄大鼠血浆LH和FSH的升高幅度减弱，MBH-LHRH的降低幅度小得多，尽管类固醇变化相当。与处于动情间期的年轻大鼠相比，老龄PD大鼠的LH和FSH水平正常，但雌二醇和孕酮浓度升高，MBH-LHRH水平降低。卵巢切除术导致MBH-LHRH正常降低；然而，促性腺激素水平的升高明显低于年轻对照组。
