Ewert D L, Paynter M J
Appl Environ Microbiol. 1980 Jan;39(1):253-60. doi: 10.1128/aem.39.1.253-260.1980.
A direct method is described for measuring bacteriophage concentrations in complex aqueous systems. Conditions for sample clarification, phage recognition, and recovery were optimized. In contrast to the plaque assay, this procedure permits quantitation of total numbers of phages independent of bacterial host. Also, the modifications increase the sensitivity of the sedimentation assay, permitting detection of particles at a minimum concentration of 10(4) per ml. Maximal total phage concentrations in the aqueous phase of sewage and activated sludge mixed liquor were 1.3 x 10(6) and 4.3 x 10(7) per ml, respectively.
描述了一种用于测量复杂水系统中噬菌体浓度的直接方法。对样品澄清、噬菌体识别和回收的条件进行了优化。与噬菌斑测定法不同，该方法允许对与细菌宿主无关的噬菌体总数进行定量。此外，这些改进提高了沉降测定法的灵敏度，允许检测最低浓度为每毫升10⁴个的颗粒。污水和活性污泥混合液水相中的最大噬菌体总浓度分别为每毫升1.3×10⁶个和4.3×10⁷个。
