对照及胰岛素处理的脂肪细胞来源质膜中D-葡萄糖转运活性与双层流动性的协同调节
Coordinate modulation of D-glucose transport activity and bilayer fluidity in plasma membranes derived from control and insulin-treated adipocytes.
作者信息
Pilch P F, Thompson P A, Czech M P
出版信息
Proc Natl Acad Sci U S A. 1980 Feb;77(2):915-8. doi: 10.1073/pnas.77.2.915.
Abstract
The cis-monoenoic fatty acids vaccenate and oleate stimulate D-glucose transport when partitioned into isolated plasma membranes from rat adipocytes. The magnitude of hexose transport stimulation due to these agents is equal to that observed in plasma membranes derived from insulin-treated adipocytes. Addition of cis-unsaturated fatty acids to plasma membranes derived from insulin-treated cells results in no further stimulation of glucose transport over that due to the hormone alone. In contrast, treatment of membranes exhibiting insulin-activated D-glucose transport activity with saturated fatty acids reduces transport activity to control levels. No effect of the saturated fatty acids was observed on D-glucose transport in control membranes. Because cis-unsaturated fatty acids fluidize plasma membranes under the conditions used in these experiments, these data demonstrate a positive correlation between membrane fluidity and adipocyte D-glucose transport system activity. In addition, the results suggest that enhanced bilayer fluidity or increased affinity of the glucose transporter for fluid microenvironments of the membrane may play a key role in transport regulation by insulin.
摘要
顺式单烯脂肪酸十八碳一烯酸和油酸在被分隔到从大鼠脂肪细胞分离出的质膜中时，会刺激D-葡萄糖转运。这些物质引起的己糖转运刺激程度与在源自胰岛素处理过的脂肪细胞的质膜中观察到的程度相同。将顺式不饱和脂肪酸添加到源自胰岛素处理过的细胞的质膜中，不会导致葡萄糖转运比仅由激素引起的转运有进一步的刺激。相反，用饱和脂肪酸处理表现出胰岛素激活的D-葡萄糖转运活性的膜会将转运活性降低到对照水平。在对照膜中未观察到饱和脂肪酸对D-葡萄糖转运有影响。由于顺式不饱和脂肪酸在这些实验所用条件下使质膜流动性增加，这些数据证明了膜流动性与脂肪细胞D-葡萄糖转运系统活性之间存在正相关。此外，结果表明，增强的双层流动性或葡萄糖转运蛋白对膜的流体微环境的亲和力增加可能在胰岛素的转运调节中起关键作用。
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