Brownstein S, Phillips T M, Lewis M G
Can J Ophthalmol. 1978 Jul;13(3):190-3.
We have identified antibodies against cytoplasmic components of allogeneic uveal melanoma cells in the sera of 16 of 31 patients with proven intraocular melanoma. Similar antibodies were found in 27% of controls and in 24% of patients with uveal nevi. The antibodies in these 3 groups of subjects were absorbed by components of uveal and cutaneous melanoma cells but not by those of normal choroidal melanocytes, normal uvea and retinal pigment epithelium, dermal nevus, pigmented skin or fetal cells. We found that specificity of the antibodies was also demonstrated by absence of reactivity with normal choroidal melanocytes, loss of reactivity after immune blocking, and absence of reactivity of melanoma antigen with conjugated antihuman immunoglobulin alone. Attempted absorption of the conjugated antihuman immunoglobulin by components of uveal melanoma cells did not alter the reactivity of the conjugated antihuman immunoglobulin.
我们在31例经证实患有眼内黑色素瘤的患者中，发现16例患者血清中存在针对同种异体葡萄膜黑色素瘤细胞胞质成分的抗体。在27%的对照者以及24%的葡萄膜痣患者中也发现了类似抗体。这三组受试者体内的抗体可被葡萄膜和皮肤黑色素瘤细胞的成分吸收，但不能被正常脉络膜黑素细胞、正常葡萄膜和视网膜色素上皮、皮肤痣、色素沉着皮肤或胎儿细胞的成分吸收。我们发现，抗体与正常脉络膜黑素细胞无反应性、免疫阻断后反应性丧失以及黑色素瘤抗原单独与偶联抗人免疫球蛋白无反应性，也证明了抗体的特异性。用葡萄膜黑色素瘤细胞成分尝试吸收偶联抗人免疫球蛋白，并未改变偶联抗人免疫球蛋白的反应性。
