Rahi A H, Garner A, Malaty A H
Br J Ophthalmol. 1978 Jun;62(6):394-401. doi: 10.1136/bjo.62.6.394.
Sera from patients with proved and suspected melanoma of the uvea and from patients with innocuous uveal naevi were found by immunohistological techniques to have an affinity for the cytoplasm of malignant choroidal melanoma cells in an appreciable proportion of cases. The responsible antibodies were subsequently shown to react with smooth muscle protein in a variety of tissues and not to be melanoma-specific. It was also shown by electron microscopy that microfilaments consistent with contractile protein are a feature of the cytoplasm of malignant choroidal melanoma cells. The latter observations, which do not appear to have been reported previously, mean that false positives are to be expected if immunodiagnostic techniques are used for the recognition of malignant disease of the uvea, especially as smooth muscle antibodies can occur in non-neoplastic ocular diseases.
通过免疫组织学技术发现，已证实和疑似患有葡萄膜黑色素瘤的患者以及患有无害葡萄膜痣的患者的血清，在相当一部分病例中对恶性脉络膜黑色素瘤细胞的细胞质具有亲和力。随后发现，相关抗体可与多种组织中的平滑肌蛋白发生反应，并非黑色素瘤特异性的。电子显微镜检查还显示，与收缩蛋白一致的微丝是恶性脉络膜黑色素瘤细胞细胞质的一个特征。后一项观察结果似乎此前未见报道，这意味着如果使用免疫诊断技术来识别葡萄膜恶性疾病，尤其是因为平滑肌抗体可出现在非肿瘤性眼部疾病中，那么就会出现假阳性结果。
