Mullen Y, Shintaku I P
Transplantation. 1980;29(1):35-42. doi: 10.1097/00007890-198001000-00008.
Survival times of allogeneic fetal pancreases were determined across various immunogenetic barriers using several inbred rat strains. Pancreases from 17-day-old embryos transplanted beneath the kidney capsule of normal, nonimmunosuppressed recipients were richly vascularized within a short period regardless of histoincompatibilities. The transplants grew progressively, increased beta cell number, and synthesized insulin at a rate similar to that in isografts until rejection intervened. Survival end points were scored by (1) complete disappearance of intrinsic vascularization and necrotic change of tissue by gross observation of grafts, (2) a sharp decrease in insulin content in a graft as compared to that in a control isograft, and (3) destruction and disappearance of beta cells in histological examination. Although fetal pancreases were rejected within 10 days in all strain combinations, there were clear differences in host immune-reactions in terms of immunogenetic barriers between donor and recipient.
使用几种近交系大鼠品系，在各种免疫遗传屏障条件下测定了同种异体胎儿胰腺的存活时间。将来自17日龄胚胎的胰腺移植到正常、未免疫抑制受体的肾被膜下，无论组织相容性如何，移植胰腺在短时间内都能充分血管化。移植胰腺逐渐生长，β细胞数量增加，并以与同基因移植相似的速率合成胰岛素，直到发生排斥反应。通过以下方式确定存活终点：(1) 通过大体观察移植物，观察内在血管化完全消失和组织坏死变化；(2) 与对照同基因移植物相比，移植物中胰岛素含量急剧下降；(3) 组织学检查中β细胞的破坏和消失。尽管在所有品系组合中胎儿胰腺在10天内均被排斥，但在供体和受体之间的免疫遗传屏障方面，宿主免疫反应存在明显差异。
