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[利用全内反射受损效应研究动物细胞与玻璃的黏附。II. 面向玻璃的细胞表面扁平化动力学]

[Use of the effect of impaired complete internal reflection for studying the adhesion of animal cells to glass. II. Kinetics of flattening of the cell surface facing the glass].

作者信息

Akatov V S, Kudriavtsev A A, Lezhnev E I, Veksler A M

出版信息

Tsitologiia. 1980 Mar;22(3):357-61.

PMID:6989062
Abstract

The kinetics of form change (flattening) of Chinese hamster fibroblast cell surface facing the glass has been studied during attachment of fibroblasts to the glass using the effect of frustrated total reflection. Two stages of the process were shown to exist. The first one is characterized by a rapid (5 min) partial flattening and requires no serum in the medium. The second stage occurs only in the presence of serum in the medium, it lasts no longer than one hour and ends in a complete flattening. After the second stage is completed, spreaded cells appear.

摘要

利用受挫全反射效应,研究了中国仓鼠成纤维细胞附着于玻璃时,其面向玻璃的细胞表面形态变化(变平)的动力学。该过程显示存在两个阶段。第一阶段的特征是快速(5分钟)部分变平,且培养基中无需血清。第二阶段仅在培养基中有血清时发生,持续时间不超过一小时,最终完全变平。第二阶段完成后,出现铺展的细胞。

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Tsitologiia. 1980 Mar;22(3):357-61.
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