Dean P N
Cell Tissue Kinet. 1980 May;13(3):299-308. doi: 10.1111/j.1365-2184.1980.tb00468.x.
Current methods of analysing DNA distributions utilize complex mathematical expressions that require the use of large non-linear curve fitting methods and, consequently, large computers. This paper presents a new method of analysing DNA distributions of asynchronously growing or mildly perturbed cells. The S phase fraction is obtained by fitting a second degree polynomial to that part of the distribution, mid S phase, which is not influenced by either the G1 or the G2 + M peaks. The method is simple and fast, it exceeds the accuracy of other methods and it can be used on a large desk calculator or mini-computer.
当前分析DNA分布的方法使用复杂的数学表达式，这需要运用大型非线性曲线拟合方法，因此需要大型计算机。本文提出了一种分析异步生长或轻度受扰细胞DNA分布的新方法。通过对分布的中间S期部分拟合二次多项式来获得S期分数，该部分不受G1期或G2 + M期峰的影响。该方法简单快速，超过了其他方法的准确性，并且可以在大型台式计算器或小型计算机上使用。
