Nenci I
Cancer Res. 1978 Nov;38(11 Pt 2):4204-11.
An experimental system designed for tracing the estradiol kinetics in target cells by specific antibodies has been applied to human breast cancer. Several major defects of the estradiol receptor mechanism have been demonstrated. The detected changes (lack of cytoplasmic receptors, impaired nuclear transfer of estradiol:receptor complexes, and abnormal nuclear retention of these complexes) have been demonstrated in most human breast cancers that appear to be composed of hormone-dependent and autonomous mixed-cell populations. These abnormalities could be the biological background for the overall or partial unresponsiveness of breast cancer to endocrine management. The participation of steroids in the regulation of centriole activities is taken into account since immunoreactive steroids are traceable by UV and electron microscopy at the level of this cell organelle by steroid antibodies. Moreover, the presence of steroids in the pericentriolar material correlates well with the modulating appearance and activity of the centriole throughout the cell cycle. A new centriole pathway is suggested by which steroid hormones can regulate cell proliferation.
一种通过特异性抗体追踪靶细胞中雌二醇动力学的实验系统已应用于人类乳腺癌。已证实雌二醇受体机制存在几个主要缺陷。在大多数似乎由激素依赖性和自主性混合细胞群体组成的人类乳腺癌中，已证实存在所检测到的变化（缺乏细胞质受体、雌二醇：受体复合物的核转运受损以及这些复合物在核内的异常滞留）。这些异常可能是乳腺癌对内分泌治疗总体或部分无反应的生物学背景。考虑到类固醇在中心粒活动调节中的作用，因为通过紫外线和电子显微镜，类固醇抗体可在该细胞器水平追踪免疫反应性类固醇。此外，中心粒周围物质中类固醇的存在与整个细胞周期中中心粒的调节性外观和活性密切相关。提出了一种新的中心粒途径，类固醇激素可通过该途径调节细胞增殖。
