放线菌根瘤和根瘤匀浆中的氢化酶。
Hydrogenase in actinorhizal root nodules and root nodule homogenates.
作者信息
Benson D R, Arp D J, Burris R H
出版信息
J Bacteriol. 1980 Apr;142(1):138-44. doi: 10.1128/jb.142.1.138-144.1980.
Abstract
Hydrogenases were measured in intact actinorhizal root nodules and from disrupted nodules of Alnus glutinosa, Alnus rhombifolia, Alnus rubra, and Myrica pensylvanica. Whole nodules took up H2 in an O2-dependent reaction. Endophyte preparations oxidized H2 through the oxyhydrogen reaction, but rates were enhanced when hydrogen uptake was coupled to artificial electron acceptors. Oxygen inhibited artifical acceptor-dependent H2 uptake. The hydrogenase system from M. pensylvanica had a different pattern of coupling to various electron acceptors than the hydrogenase systems from the alders; only the bayberry system evolved H2 from reduced viologen dyes.
摘要
在完整的放线菌根瘤以及来自欧洲桤木、菱形桤木、红桤木和宾夕法尼亚杨梅的破碎根瘤中测定了氢化酶。完整的根瘤在一个依赖氧气的反应中吸收氢气。内生菌制剂通过氢氧反应氧化氢气，但当氢气吸收与人工电子受体偶联时，速率会提高。氧气抑制依赖人工受体的氢气吸收。宾夕法尼亚杨梅的氢化酶系统与桤木的氢化酶系统相比，与各种电子受体偶联的模式不同；只有杨梅系统能从还原的紫精染料中释放氢气。
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