Benson D R, Arp D J, Burris R H
J Bacteriol. 1980 Apr;142(1):138-44. doi: 10.1128/jb.142.1.138-144.1980.
Hydrogenases were measured in intact actinorhizal root nodules and from disrupted nodules of Alnus glutinosa, Alnus rhombifolia, Alnus rubra, and Myrica pensylvanica. Whole nodules took up H2 in an O2-dependent reaction. Endophyte preparations oxidized H2 through the oxyhydrogen reaction, but rates were enhanced when hydrogen uptake was coupled to artificial electron acceptors. Oxygen inhibited artifical acceptor-dependent H2 uptake. The hydrogenase system from M. pensylvanica had a different pattern of coupling to various electron acceptors than the hydrogenase systems from the alders; only the bayberry system evolved H2 from reduced viologen dyes.
在完整的放线菌根瘤以及来自欧洲桤木、菱形桤木、红桤木和宾夕法尼亚杨梅的破碎根瘤中测定了氢化酶。完整的根瘤在一个依赖氧气的反应中吸收氢气。内生菌制剂通过氢氧反应氧化氢气，但当氢气吸收与人工电子受体偶联时，速率会提高。氧气抑制依赖人工受体的氢气吸收。宾夕法尼亚杨梅的氢化酶系统与桤木的氢化酶系统相比，与各种电子受体偶联的模式不同；只有杨梅系统能从还原的紫精染料中释放氢气。
