一种用于证明旨在限制冠状动脉闭塞后梗死面积的干预措施疗效的方法：透明质酸酶的有益作用。

A method for demonstrating the efficacy of interventions designed to limit infarct size following coronary occlusion: beneficial effect of hyaluronidase.

作者信息

Riberio L G, Hillis L D, Louie E K, Davis M A, Maroko P R, Braunwald E

出版信息

Cardiovasc Res. 1978 Jun;12(6):334-40. doi: 10.1093/cvr/12.6.334.

DOI:10.1093/cvr/12.6.334

Abstract

In order to measure the protective effect of interventions following coronary artery occlusions in dogs, the creatine kinase activity of myocardial tissue was assayed after 24 h and related to the myocardial blood flow of that tissue measured with 85Sr labelled microspheres injected 15 min after occlusion. This assay showed normal levels when flow exceeded 50 cm3.min-1.100 g-1. In myocardium with flow reduced to 0 to 15 cm3. min-1.100g-1, creatine kinase activity was 7.6 +/- 0.6 IU.mg-1 protein in control dogs and 13.1 +/- 1.8 IU.mg-1 protein (P less than 0.01) in dogs given 500 NF units.kg-1 of hyaluronidase 20 min after occlusion. Where myocardial blood flow was reduced to 16 to 50 cm3. min-1. 100g-1, creatine kinase activity was increased from 14.1 +/- 1.1 to 20.5 +/- 1.4 IU.mg-1 protein by hyaluronidase. This method therefore assesses ischaemic damage independent of electrophysiological measurements and confirms myocardial preservation by hyaluronidase.

摘要

为了测定犬冠状动脉闭塞后干预措施的保护作用，在24小时后测定心肌组织的肌酸激酶活性，并将其与闭塞15分钟后注射85Sr标记微球所测量的该组织心肌血流量相关联。当血流量超过50 cm³·min⁻¹·100g⁻¹时，该测定显示正常水平。在血流量降至0至15 cm³·min⁻¹·100g⁻¹的心肌中，对照犬的肌酸激酶活性为7.6±0.6 IU·mg⁻¹蛋白质，而在闭塞后20分钟给予500 NF单位·kg⁻¹透明质酸酶的犬中为13.1±1.8 IU·mg⁻¹蛋白质（P<0.01）。当心肌血流量降至16至50 cm³·min⁻¹·100g⁻¹时，透明质酸酶使肌酸激酶活性从14.1±1.1增加至20.5±1.4 IU·mg⁻¹蛋白质。因此，该方法可独立于电生理测量来评估缺血损伤，并证实透明质酸酶对心肌的保护作用。