Fontaine M, Joisel F, Dumouchel L
J Immunol Methods. 1980;33(2):145-58. doi: 10.1016/s0022-1759(80)80005-6.
We have devised a simple one-step preparation of C3 depleted serum (R3). Fresh normal human serum, with complement activation inhibitors, was depleted of C3 by affinity chromatography on a Sepharose anti-C3c. To prevent non-specific interactions, a high ionic strength buffer containing saccharose was used. Immunochemical analysis and complement activity assays demonstrate that this C3 depleted serum is a suitable R3 reagent. This R3 reagent has been used successfully for titration of hemolytic activity of C3 in normal and abnormal sera. Two simple, specific and sensitive assays are described.
我们设计了一种简单的一步法制备C3缺失血清（R3）的方法。含有补体激活抑制剂的新鲜正常人血清，通过在琼脂糖抗C3c上进行亲和层析去除C3。为防止非特异性相互作用，使用了含有蔗糖的高离子强度缓冲液。免疫化学分析和补体活性测定表明，这种C3缺失血清是一种合适的R3试剂。该R3试剂已成功用于滴定正常和异常血清中C3的溶血活性。描述了两种简单、特异且灵敏的测定方法。
